Purification and characterization of two PR-10 protein isoforms from the crude drug of Angelica sinensis / 生物工程学报

Two proteins of similar molecular weight (named as ASPR-C-1 and ASPR-C-2) from the crude drug of Angelica sinensis were purified and characterized by 80% ammonium sulfate precipitation, Sephadex G-50 gel filtration chromatography, and DEAE-Sepharose anion exchange chromatography. The molecular weight of ASPR-C-1 and ASPR-C-2 on SDS-PAGE was 17.33 kDa and 17.18 kDa, respectively. They were mainly monomeric in solution, but partially formed dimers and they were glycoproteins with glycosyl content of 2.6% and 8.2%, respectively. Both ASPR-C-1 and ASPR-C-2 were identified to be members of pathogenesis-related 10 family of proteins by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and have ribonuclease activities with the specific activity of 73.60 U/mg and 146.76 U/mg, respectively. The optimum pH of the two isoforms was similar, at about 5.6, while their optimum temperatures were different. The optimum temperature of ASPR-C-1 was 50 ℃, and that of ASPR-C-2 was 60 ℃. Both isoforms presented highest thermal stability at 60 ℃. However, ASPR-C-2 was more thermotolerant than ASPR-C-1. The latter was rapidly inactivated and retained only about 20% residual activity while the former still maintained about 80% of its original activity at a higher treatment temperature (80 to 100 ℃). In addition, Fe²⁺ had an activating effect on the ribonuclease activities of two isoforms while Ca²⁺, Mg²⁺, Zn²⁺, Mn²⁺, Ag⁺, Cu²⁺, EDTA (Elhylene diamine tetraacetic acid), dithiothreitol and sodium dodecylsulphate showed different degrees of inhibition of the enzyme activities. Our findings provide a foundation for further research on the biological function of PR-10 protein from Angelica sinensis.

Efficacy of "tennis racket"-like flap with retrograde medial plantar on repairment of frontal plantar tissue defects / 中华医学美学美容杂志

Objective To explore the clinical experience and surgical method of the repairment of frontal plantar tissue defects by using "tennis racket"-like flap with the medial plantar retrograde,and to study the reliability in the clinical application of the medial plantar retrograde flap.Methods From June 2011 to June 2016,"tennis racket"-like flap with the medial plantar retrograde was used to repair the frontal plantar tissue defects in 10 cases.The cutting range of flap was from 3.5 cm × 2.0 cm to 8.0 cm x 4.0 cm in size;in all patients the donor area was covered by skin grafts.Results All flaps survived and wounds healed by first intention.In 10 patients the donor sites healed primarily with a straight scar,and the appearance and texture of the flaps were satisfactory.All patients were followed up from 6 to 24 months (mean 12 months).According to the Chinese foot function evaluation standard trial evaluation,the outcomes were excellent in 9 cases,good in 7 cases,and medium in 2 cases.Conclusions "Tennis racket"-like flap with the medial plantar retrograde is less anatomic variation with reliable blood supply,and sensory recovery is quick;the donor site is a small crater and cicatrial contractures are light;the cost is low.All patients are treated on one session and therefore it is an ideal method for the repairment of frontal plantar tissue defects.

Preparation of immunotoxin BI7D12-PE38KDEL directed to EGFR and determination of its activity in vitro / 中国免疫学杂志

Objective:To prepare nanobody-based immunotoxin BI7D12-PE38KDEL targeting EGFR and to examine its cytotoxicity against EGFR positive tumor cells.Methods:By using molecular cloning strategy,prokaryotic expression construct of pET28a-BI7D12-PE38KDEL was generated which consisted of nanobody 7D12 targeting EGFR in the form of a divalent fused with PE38KDEL,a truncated form of pseudomonas exotoxin A via a flexible peptide(G4S)4,and then transformed into E.coli BL21(DE3).Protein expression was induced by adding IPTG,purified by Ni-affinity column chromatography,and verified by Western blot.The binding capacity of the resulted immunotoxin to EGFR-positive cells A549,HT29,MCF-7 and EGFR-negative cells CEM,Jurkat were determined by flow cytometry assay,and its cytotoxicity against the target cells was examined.Briefly,tumor cells were treated with different dosage of the immunotoxin,and the killing efficacy of BI7D12-PE38KDEL on these cells were assessed by WST-1 assay after 72 hours.Results:The SDS-PAGE and Western blot results showed the recombinant immunotoxin BI7D12-PE38KDEL was successfully prepared,and majority of them was expressed in soluble form.BI7D12-PE38KDEL could selectively bind to EGFR-positive cells of A549,HT29,and MCF-7.More importantly,the immunotoxin exhibited much more significant killing effect on these EGFR positive cells compared to the negative control group of CEM and Jurkat cells(P<0.01).Conclusion:In the current study,the nanobody-based immunotoxin BI7D12-PE38KDEL targeting EGFR was successfully prepared and exhibited a superior inhibition effect for the growth of EGFR-positive cells.

Expression, purification, stability and transduction efficiency of full-length SOD2 recombinant proteins / 生物工程学报

Superoxide dismutase (SOD) family is necessary to protect cells from the toxicity of reactive oxygen species produced during normal metabolism. Among SODs, manganese-containing superoxide dismutase (Mn-SOD, SOD2) is the most important one. The DNA fragment containing the full nucleotide of full-length human SOD2 was synthesized and inserted into the prokaryotic expression vector pGEX-4T-1 with tag GST. DNA construct was then transformed into Escherichia coli BL21 (DE3) and expression was induced with IPTG at 25 ℃. The recombinant fusion protein GST-SOD2 (46 kDa) was purified from the bacterial lysate by GST resin column affinity chromatography. GST tag was cleaved with thrombin, and a crude SOD2 recombinant protein (25 kDa) was obtained and further purified by heparin affinity chromatography. Activities of the two SOD2 proteins were 1 788 and 2 000 U/mg, respectively. Both SOD2 proteins were stable under physiological condition and cell-penetrating (P<0.05). Our findings open the possibility to study the structure and effects of two full-length recombinant SOD2 proteins.

Analysis of Intestinal Microbiota of Type 2 Diabetes Patients of by Two Fingerprint Technologies / 现代检验医学杂志

Objective To explore the characteristics of intestinal Microbiota in T2DM patients by two molecular fingerprint technologies,and investigate the correlation of intestinal microbiota and T2DM,and evaluate the application value of two fin-gerprint technologies.Methods Fecal samples of 8 healthy groups and 7 diabetes patients were collected.Then the total DNA of gut microbiota was extracted.Through the analysis of products by two molecular fingerprints of ERIC-PCR and DGGE-PCR,ecological characteristics of diversity and similarity of gut microbiota were obtained in healthy groups and dia-betes patients.Results Compared to healthy groups,the number of bands and Shannon-Wiener index of gut microbiota in di-abetes patients was decreased but no statistical significance.The similarity in patients group was declining(P <0.05),and the construction of gut microbiota was inclined to differ.Two fingerprint technologies of ERIC and DGGE could directly re-flect the diversity of gut microbiota and were the modern molecular biological techniques without depending on cultivation. ERIC was simple and convenient,had a better reflection of microbial diversity,but gel band cutting and regarded asa proper approach with higher diffraction efficiency and excellent repetition to studysequencing couldn’t be performed since there were more influencing factors on the experiment.DGGE could better reflect the ecological characteristics such as microbial diversity and similarity,and selecting bands,gel band cutting and sequencing could be done.Conclusion The composition and construction of gut microbiota in diabetes patients were changed,which suggests the occurrence of the disease had the correlation with gut microbiota.ERIC and DGGE is regarded as a proper approach with higher diffraction efficiency and ex-cellent repetition to study intestinal microbiota,but also gel band cutting,sequencing,bacteria identification can be performed by DGGE,both can be used in combination.

Reality of Negative anti-HBc and Positive anti-HBe Results of Testing HBV-M with ELISA / 现代检验医学杂志

Objective To research the reality of ELISA testing results with negative anti-HBc and positive antiHBe.Methods CMIA was carried out to retest antiHBc and antiHBe of 105 samples which were initially tested to have negative antiHBc and positive antiHBe.Results Among the 105 samples retested by CMIA,there were three different results,positive antiH-Bc with positive antiHBe,negative antiHBc with negative antiHBe and positive antiHBc with negative antiHBe,whose pro-portions were 72.38%,21.91% and 5.71% respectively;the fasle negative rates of antiHBc were 78.10% in total and 93.33%,96.15% and 47.37% in 3 subgroup with S/CO 1.00~ 1.20,1.20~2.00 and 2.00~ 3.00,respectively;the true positive rates of antiHBe were 72.38% in total and 42.86%,88.14% and 56.25% in 3 subgroups with S/CO 0.00~0.10, 0.10~0.80 and 0.80 ~ 1.00,respectively.Conclusion HBV-M results with negative antiHBc and positive antiHBe by ELISA could give suggestive value and not reflect true information about antiHBc and antiHBe with three alternatives which would be obtained through retesting by a second assay.

Study on the changes of peripheral blood lymphocyte scatter diagram in patients newly diagnosed with hepatitis B / 国际检验医学杂志

Objective To study the clinical significance of leukocyte parameters measured by using automatic blood cell analyzer SysmexXE‐2100inpatientsnewlydiagnosedwithhepatitisB.Methods 96patientsnewlydiagnosedwithhepatitisB(observation group) and 100 people who underwent healthy examination during the same period(control group) were recruited in the study .Rel‐ative indicators were measured for the people mentioned above ,such as the DNA of Hepatitis B virus(HBV‐DNA) ,Hepatitis B sur‐face antigen(HBsAg) ,aspartate aminotransferase(AST ) ,alanine aminotransferase(ALT ) ,lymphocytes parameters Lymph‐Y and Lymph‐X ,neutrophils parameters Neut‐X and Neut‐Y .The test results were recorded and statistically analyzed by using software SPSS19 .0 .Results The differences of Lymph‐X ,Neut‐Y and Neut‐X were not statistically significant compared between observa‐tion group and control group(P>0 .05) ,while the differences of HBV‐DNA ,HBsAg ,AST ,ALT and Lymph‐Y were statistically significant compared between the two groups(P<0 .05) .In addition to that ,the parameter Lymph‐Y was positirely correlated with HBV‐DNA(r=0 .160 ,P=0 .026) and HBsAg(r=0 .149 ,P=0 .037) .Conclusion The peripheral blood lymphocytes parameter Lymph‐Y of patients newly diagnosed with hepatitis B is higher than healthy people ,which makes it possible to become an indicator for differential diagnosis .

The application of the joint detection of mALB,α1-M and NAG in the diabetes and hypertension patients with early injury of kidney / 国际检验医学杂志

Objective To explore the diagnosis value of joint detection of microalbumin(mALB) ,α1-Microglolin(α1-M ) and N-acety-β-D-glucosaminidase(NAG) in the diabetes and hypertension patients with early injury of kidney .Methods Sample were col-lected from July 2013 to January 2014 ,including 63 diabetic cases(diabetic group) ,58 patients with hypertension(hypertension group) and 64 health controls(control group) ,then the levels of urinary mALB ,α1-M were detected by immunoturbidimetry ,urina-ry NAG activity was assessed by endpoint colorimetric assay .Results The levels of urinary mALB ,α1-M and NAG in diabetic group and hypertension group were higher than those in control group(P<0 .05) .The positive rates of three indices single detected were less than 50 .0% ,the positive rates of any two indices joint detected were more than 50 .0% ,the positive rate of three indices joint detected was more than 70 .0% .Conclusion The method of urinary mALB ,α1-M and NAG joint detected is sensitive and reli-able for diagnosing of the early injury of kidney .

Study on conformance between two kinds of detection method of anti-HBs antibody / 国际检验医学杂志

Objective To analyze the conformance between the quantitative and qualitative tests of hepatitis B surface antibody (anti-HBs).Methods The chemiluminecence microparticle enzyme immunoassay(CMIA)was adopted to quantitatively detect anti-HBs and the enzyme linked immunosorbent assay(ELISA)was adopted to qualitatively detect anti-HBs.Results With the CMIA as the reference experiment,Se ,Sp ,J ,PV+ and PV-of anti-HBs detected by ELISA were 0.95,0.53,0.48,0.74 and 0.88 respec-tively,k=0.51;when the absorbance was 0.400 9,Se ,Sp ,J ,PV+ and PV-were 0.50,0.95,0.45,0.93 and 0.43 respectively;for the samples exceeding the absorbance range of 0.104 3 -0.400 9,Se ,Sp ,J ,PV+ and PV-qualitatively detected by ELISA were 0.90,0.91,0.81,0.93 and 0.88 respectively,k =0.81.Conclusion Determining cutoff value with the absorbance value 0.105 as the ELISA detection result has the good detection sensitivity(Se =0.95)and the better negative prediction value(PV-=0.88),the negative anti-HBs detected by ELISA may be considered that the anti-HBs concentration was less than 10 mIU/mL without the conservation value;when the sample absorbance ≥0.400 9,the anti-HBs concentration is ≥10 mIU/mL,which may be considered to have the conservation value;the gray area range of anti-HBs detected by ELISA is mainly the absorbance of 0.105-0.400 9,the true anti-HBs level should be quantitatively detected.

Study and analysis on the quantitive detection of EBV-DNA in adenoidal hypertrophic and tonsillitis tissues of children / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the epidemiology of EBV in adenoidal hypertrophy and chronic tonsillitis and discuss the affection of EBV on the nosogenesis of adenoidal hypertrophy and tonsillitis of children.@*METHOD@#Fifty-two children with chronic tonsillitis and/or adenoidal hypertrophy had the operations of the tonsillectomy and/or the adenoidectomy. These tissues resected and plasma of all cases were detected to find EBV-DNA by RQ PCR.@*RESULT@#The infection rate of EBV in the tissues of adenoidal hypertrophy and tonsillitis of children was 51.9%. The boys' infection rate of EBV was 50.0%, and the girls' infection rate of EBV was 55.6%, which had not significantly different. The EBV infection rate in the tissues of tonsillitis was 40.4%, The EBV infection rate in the tissues of adenoidal hypertrophy was 48.9%, which had not significant difference. The school age group (7- to 14-years-old) presented higher infection rate of EBV in the tissues of adenoid and tonsil (65.5%) than the pre-school children group (2- to 6-years-old) (34.8%). Comparing the copies numbers of EBV-DNA in the different degrees of adenoidal hypertrophy, we found that the copies numbers of EBV-DNA in the severe hypertrophy group were higher than the midrange and slight hypertrophy groups (P<0.05). Meanwhile we detected EBV-DNA in these childrens' blood plasma by RQ-PCR. No blood plasma was detected EBV-DNA copies higher than normal (< 1 x 10(3) copies/ml).@*CONCLUSION@#The tissues of adenoidal hypertrophy and tonsillitis had same sensitivity to EBV. There was not significant difference between the infection rates of the boys and girls with adenoidal hypertrophy and/or tonsillitis. With these children growing up and the course of diseases prolonging, the infection rate of EBV increased correspondingly. There was a certain correlation between the hypertrophy of adenoid and EBV. There were no EBV-DNA fragments in blood plasma of the children with adenoidal hypertrophy and/or tonsillitis. So there were essential different between benign hyperplasia and nasopharyngeal carcinoma.

Study and analysis on the quantitive detection of EBV-DNA in adenoidal hypertrophic and tonsillitis tissues of children / 临床耳鼻咽喉头颈外科杂志

Objeetive:To investigate the epidemiology of EBV in adenoidal hypertrophy and chronic tonsillitis and discuss the effection of EBV on the nosogenesis of adenoidal hypertrophy and tonsillitis of children.Method:Fifty-two children with chronic tonsillitis and/or adenoidal hypertrophy had the operations of the tonsillectomy and/or the adenoidectomy.These tissues reseeted and plasma of all cases were detected to find EBV-DNA by RQPCR.Result:The infection rate of EBV in the tissues of adenoidal hypertrophy and tonsillitis of childen was 51.9%.The boys'infection rate of EBV was 50.0%,and the girls'infection rate of EBV was 55.6%,which had not significantly different.The EBV infection rate in the tissues of tonsillitis was 40.4%,The EBV infection rate in the tissues of adenoidal hypertrophy was 48.9%,which had not significant difference.The school age group(7to 14 years old)presented higher infection rate of EBV in the tissues of adenoid and tonsil(65.5%)than the preschool children group(2 to 6 years old)(34.8%).Comparing the copies numbers of BV-DNA in the different degrees of adenoidal hypertrophy,we found that the copies numbers of EBV-DNA in the severe hypertrophy group were higher than the midrange and slight hypertrophy groups(P＜0.05).Meanwhile we detected EBV-DNA in these childrens'blood plasma by RQ-PCR.No blood plasma was detected EBV-DNA copies higher than normal(＜1×10~3 copies/ml).Conclusion:The tissues of adenoidal hypertrophy and tonsillitis had same sensitivity to EBV.There was not significant difference between the infection rates of the boys and girls with adenoidal hypertrophy and/or tonsillitis.With these children growing up and the course of diseases prolonging,the infection rate of EBV increased correspondingly.There was a certain correlation between the hypertrophy of adenoid and EBV.There were NO EBV-DNA fragments in blood plasma of the children with adenoidal hypertrophy and/or tonsillitis.So there were essential different between benign hyperplasy and nasopharyngeal carcinoma.

Modulation of the adhesion of neutrophils to endothelium and the expression of intercellular adhesion molecule 1 in endothelial cells by one small peptide interfering with IgG-FcγR interaction / 中华肾脏病杂志

Objective To investigate the effects of tg19320,a small peptide,interfering with IgG-FcγR interaction on the adhesion of neutrophils to endothelium and the expression of intercellular adhesion molecule 1 (ICAM-1)in endothelial cells and its possible mechanism.Methods Tg19320 was prepared by solid-phase peptide synthesis.ANCA IgG was isolated from the serum of active ANCA-associated systemic vasculitis(AASV)patients.When primary human umbilical vein endothelial cells(HUVEC)grew into connuence in cytokine-free eonditions,the cells were stimulated with TNF-α,human normal IgG,ANCA IgG and ANCA IgG+tg19320 respectively.HUVEC were pretreated with tg19320 for 45 minutes before being stimulated by ANCA IgG.Non-activated neutrophils was added to treat HUVEC and adhesion was measured by cell count.The expression of ICAM-1 mRNA and protein was assessed by real-time PCR and Western blot respectively.Soluble ICAM-1(sICAM-1)was determined using ELISA technique.Phosphorylation of IκB-α was assessed by Western blot. Results ANCA IgG significantly up-regulated the expression of ICAM-1 in HUVEC and promoted sICAM-1 release(P＜0.05),and TNF-α enhanced the effect of ANCA.These effects were almost completely abolished by tgl9320 both at protein and mRNA level.Furthermore,ANCA IgG increased the IκB-α phosporylation in HUVEC and tg19320could inhibit the effect. Conclusions ANCA IgG can modulate the expression of ICAM-1 and sICAM-1 release in endothelial cells.FcγR probably play a critical role in the ICAM-1 expression up-regulated by ANCA,which is mediated in part through NF-κB signaling pathway.Tg19320 has protective effect on endothelium in AASV in vitro.

The expression of neutrophils FCγRⅡ/Ⅲ modulated by anti-neutrophil cytoplasmic antibodies and its clinical value / 中华风湿病学杂志

Objective The interaction between anti-neutrophil cytoplasmic antibaties (ANCA) and receptors at the Fc portion of immunoglobulins (FeγR) is central in the pathogenisis of primary systemic small vasculitis. The aim of this study is to investigate the role and clinical value of ANCA on the expression of neutrophils FCγRⅡ/Ⅲ (CD32/CD16). Methods ANCA IgG was prepared from the sera of patients with active We-gener's granulomatosis (WG) and microscopic polyangiitis (MPA). Neutrophils were isolated from the blood of healthy volunteers. The expression of CD32/CD16 on neutrophils was assessed by flow cytometry after stimulated by ANCA for 1 hour. We compared the expression of CD32/CD16 between 18 primary systemic small vaseulitis (PSV) patients and 35 healthy volunteers. Furthermore, the correlation was also be analyzed between the expression of CD32/CD16 and Birmingham vasculitis activity score (BVAS). Results The expression of CD16 was significandy elevated by ANCA (Mnx 67±23 vs 54±21, P<0.01 ). The expression of CD16 was higher in patients than in healthy volunteers (Mnx 62±12 vs 53±10, P<0.01), which was in correlation with BVAS (r=0.728 86, P<0.01). But no such correlation was found for CD32 . Conclusion ANCA may play a role in the pathogenesis of PSV by modulating the expression of the FCγR. Monitoring the expression of CD16 on neutrophils is helpful for the evaluation of PSV activity.

Inhibitory effect of an small peptide able to interfere with Fc?-receptor recognition on ANCA-accelerated apoptosis of neutrophils / 中华肾脏病杂志

Objective To investigated the inhibitory effect of an IgG-Fc region specific inhibitory peptide on the ANCA-accelerated apoptosis of neutrophils. Methods The peptide was prepared by solid-phase peptide synthesis and its biological activity was identified by rosette formation assay. ANCA was prepared from the sera of active Wegener's granulomatosis (WG) and microscopic polyangiitis (MPA) patients. Neutrophils isolated from the blood of healthy volunteers were primed with TNF-?(2 ng/ml) then incubated with ANCA. At different intervals(3, 6, 12, 18 hours) the neutrophils were harvested to assess the apoptosis by flow cytometric analysis of JC-1 staining, Sub-G1 population and fonnation TUNEL technique. Results Tg19320 bound tightly to human IgG dose-dependendy and inhibited statistically the rosette formation between SRBC-IgG and U937 cells(20.3% vs 53.2% ,P

Construction of sense and antisense eukaryotic expression vector of novel gene Collectrin and its function in cell growth / 北京大学学报(医学版)

Objective: To construct sense and antisense eukaryotic expression vector of novel gene Collectrin and identify its function in cell growth. Methods: The open reading frame of Collectrin was amplified by PCR and inserted into pcDNA3.1/V5 His plasmid. The recombinant plasmid was identified by restriction enzyme analysis and sequencing analysis. The recombinant plasmid was transfected into M 1 cell by using lipofectin mediation after being identified by restriction enzyme analysis and sequencing analysis. RT PCR and Western blot were performed to identify the expression of Collectrin. ? Gal staining was used to define the effect of tansfection .The growth of M 1 cells was examined by MTT and cell counting. Results: Compared with control group, the expression of Collectrin was decreased significantly at both nucleotide and protein levels tansfected by antisense vector, but elevated in sense group. The cell growth was blocked after being transfected by antisense plasmid. Conclusion: The sense and antisense eukaryotic expression vector of novel gene Collectrin was successfully constructed. Collectrin was one of basic factors in cell growth.

THE DISTRIBUTON CHARACTERISTIC OF FIVE PEPTIDE HORMONES IN THE ENDOCRINE CELLS IN DIGESTIVE TRACT OF MONOPTERUS ALBUS / 解剖学报

Objective To investigate the localization and distribution of the five endocrine cells in the digestive tract mucosa of ricefield eel(Monopterus albus). Methods Using immunocytochemical technique of strept avidin-biotin-peroxidase complex(SABC) were used. Results At least 5 kinds of immunoreactive endocrine cells distributed in the digestive tract mucosa of M.albus. They were gastrin(Gas),somatostatin(Som),5-hydroxytryptamine(5-HT),insulin(Ins),and neurofilament (NF) immunoreactive endocrine(IRE) cells.Gas and Som-IRE cells distributed between stratified squamous epithelium and goblet cell in esophagus. A large number of Gas-IRE cells were found between gastric fundus epithelium and gastric glands, and only a few in the carcia. Ins, 5-HT and NF-IRE cells distributed in the epithelium pylorus and pyloric glandular tube respectively. No any immunoreactive positive reaction was found in the gut of M.albus.In addition, immunoreactive positive reaction of glucagons was not found in whole digestive tract.All immunoreactive endocrine cells were dark brown in color.Their morphology was irregular, cytoplasmic process was shorter and thicker, their nucleus showed an empty bubble.They distributed between esophageal epithelium and gastric epithelium or glandular epithelium, and cytoplasmic process extended to the gastric lumen and glandular cavity.Conclusion There is a complex endocrine function of the digestive system in ricefield eel (M.albus) at the lowest vertebrate.

Study of the genotype of extended-spectrum ?-lactamases in Enterobacteriaceae of Xi'an city / 西安交通大学学报(医学版)

Objective To study the genotypes of ESBL-producing Enterobacteriaceae in Xi'an city.Methods Totally 125 ESBL-producing Enterobacteriaceae strains were randomly selected from five hospitals of Xi'an City,and TEM-type,SHV-type and CTX-M-type ESBL genes were amplified by PCR.Results Among the 125 ESBL-producing Enterobacteriaceae strains,TEM-type ESBL genes were amplified from 33 strains,SHV-type ESBL genes were amplified from 26 strains,CTX-M-type ESBL genes were amplified from 49 strains,and two or more type ESBL genes were amplified from 22 strains.Conclusion CTX-M-type ESBL are prevalent in ESBL-producing Enterbacteriaceae in Xi'an City.

Mechanism of the change of blood pressure in rat model of high-fat diet-induced obesity / 西安交通大学学报(医学版)

Objective To investigate the influence of high-fat diet on blood pressure and metabolism in Sprague-Dawley(SD) rats and its mechanism.Methods Forty male SD rats were randomly divided into 4 groups which were fed with a diet containing 53% calorie as fat(HF) or a normal diet(ND) for 5 or 10 weeks.Systolic blood pressure(SBP),body weight,abdominal adipose tissue,blood lipids,fasting insulin(FINS),and fasting plasma glucose(FPG) were measured after 5 and 10 weeks respectively.Results SBP of HF groups were higher than that of ND groups [HF5 vs.ND5,(105.506?4.634)mmHg vs.(100.060?4.773)mmHg,P

STUDY OF LYMPHOCYTE SUBSETS AND FUNCTION OF THE SUPPRESSIVE T LYMPHOCYTE IN PATIENTS WITH VIRAL MYOCARDITIS / 西安交通大学学报(医学版)

The frequency of peripheral lymphocyte subsets using monoclonal was studied in patients with viral myocarditis (MC). The percentage of T_8 positive cells was significantly lower in patients with MC of heart failure(MCHF) than that in patients with MC of arrhythmias and that in normal controls, whereas the T_4/T_8 ratio was significantly inereased in MCHF patients. At the same time, suppressive T cells (Ts) function in patients with MC was evaluated by shortlived assay. The Ts function was significantly reduced in MCHF patients, close correlation was found between the Ts function and the hemodynamic function in MCHF patients. Abnormal immunoreguiation may be of the important pathogenic factor in MCHF patients.

Comparison of the Cox-2 expression in esophageal, gastric and cardia carcinomas / 西安交通大学学报(医学版)

Objective To determine whether Cox-2 inhibitor can reduce the risk of cardia carcinoma. Methods Paraffin-embedded specimens from 48 patients with esophageal, gastric or cardia carcinomas were analyzed by immunohistochemistry for expression of Cox-2 protein. Expression of Cox-2mRNA was assessed by RT-PCR and ISPCR in 29 cases of them. None of these patients were currently taking NSAIDs or glucocorticoid. Results The staining scores were 4.15?1.9 in the group with esophageal cancer, 3.66?1.16 in the group with gastric cancer, and 2.93?1.03 in the group with cardia cancer, respectively. There was no significant difference between groups of gastric cancer and cardia cancer. The ratio of cases with positive expression of Cox-2 mRNA was 87.5% in the group with cardia carcinoma, 100% in the group with esophageal cancer and the group with gastric cancer. And no significant difference was found between them. Cox-2mRNA was mainly located in cytoplasm but was found in nuclear too. No difference was found in the location of Cox-2 expression in the three kinds of cancers. Conclusion Cox-2 expression in cardia carcinoma was higher than in the normal group. Its pathological characteristics were almost the same as those in gastric and esophageal cancers. Cox-2 inhibitor possibly have a chemopreventive effect on cardia carcinoma.